The in-situ method of conservation refers to the preservation of species in their natural habitats, ensuring that ecosystems remain intact and functional. This technique involves protecting whole ecosystems and their biodiversity, such as through national parks and wildlife sanctuaries. In contrast, a botanical garden is typically a managed and cultivated space for the ex-situ conservation of plant species, where plants are grown outside their natural habitats for purposes such as research, education, and conservation. Therefore, the correct option is "Botanical Garden," as it does not align with the definition of in-situ conservation.